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Stacking and subsequent elution with the analyte bands in FASS calls for an EOF velocity a little bit bigger compared to the electrophoretic velocity with the target analytes and in the other direction. Cellulose and glass fiber papers inherently have negative surface charge, producing them suitable for EK stacking of negatively billed analytes. Stacking of cationic analytes demands a favourable area cost, which we imparted utilizing amino-made up of silanizing reagents as explained from the “Paper Modification” part (supporting details). Exact control of EOF using chemical modifications was laborious, on the other hand. Insufficiently large ζ-potentials resulted in diffuse stacking bands and analyte transport far from the ionization zone. Abnormal ζ-potentials launched noticeable turbulence and instability inside the stacking bands.
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Lower fees for each examination by considerably cutting down solvent consumption and disposal, removing chromatography upkeep costs; additionally, run a lot more exams in residence.
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